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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Theriogenology. 2015 Jun 12;84(6):1024–1032. doi: 10.1016/j.theriogenology.2015.05.036

Figure 2.

Figure 2

Target of TPEN is Zn2+ specific. (A) Mature oocytes incubated with TPEN have a lower concentration of intracellular Zn2+ compared to the control group. (B) Quantification of the level of Zn2+ in mature oocytes treated with TPEN and the control. Intensity of fluorescence was measured by Image J (n=8 in each group). The difference was significant (p<0.05) (C) Presence of exogenous Zn2+ inhibits oocyte activation induced by TPEN. Pronuclear formation after TPEN treatment was dramatically reduced with the addition of Zn2+.