Table 4.

Effect of TPEN as a main oocyte activating reagent. Different letters indicate statistical difference (p<0.05).

Treatment	Number of embryos	Day 6 Blastocysts (%)	Day 7 Blastocysts (%)
Control - electroporation	80	13.75^a	17.5^a
10 μM, 30 min	40	0^b	5^b
10 μM, 1 hour	40	15^a	22.5^a
10 μM, 2 hours	40	15^a	25^a
100 μM, 10 min	40	15^a	15^a
100 μM, 20 min	40	25^a	30^a
200 μM, 30 min	81	37.04^c	41.98^c
200 μM, 1 hour	80	18.75^a	27.5^a