Fig. 2.
Activation of STAT6 by IL-4 is diminished but not abrogated in γc-deficient macrophages. a Bone marrow-derived macrophages from control (wild-type) and γc KO (Il2rg-/-) mice (C57BL/6J background) were treated with log10 concentrations of IL-4 ranging from 1,000 to 0.1 ng/ml for 30 min at 37°C. An additional set of bone marrow-derived macrophages from γc KO mice were treated with IL-4 in the presence of the neutralizing anti-IL-4Rα1 mAb, M1 (lanes 13–18). Nuclear protein extracts were then prepared and the levels of activated STAT6 were measured by EMSA using a radiolabeled SBE1 probe. b Another set of macrophages derived from the same mice were incubated with IL-13 (1,000-10 ng/ml) for 30 min at 37°C (lower panel). Nuclear protein extracts were then prepared and analyzed for STAT6 activity by EMSA using the SBE1 probe.