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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Nat Cell Biol. 2015 Aug 10;17(9):1134–1144. doi: 10.1038/ncb3223

Figure 2. The kinetochore core remains relatively compact throughout mitosis.

Figure 2

(a,d) Maximal intensity projections (include all kinetochores) depicting RPE1 cells at various stages of mitosis. (b,e) Examples of individual kinetochores from the boxed areas in (a) and (d) shown at higher magnification. The shape of Hec1 (b, red) and Mis12 (d, red) distributions remains relatively compact. Notice that the amount of Hec1 increases significantly (c) while the amount of Mis12 and the volume occupied by this protein remain constant (f,g). Also notice that the shape of inner kinetochore remains compact throughout mitosis (green, CenpA-GFP). Blue bars in (c), (f), and (g) are calculated as mean of mean values for multiple kinetochores in individual cells (n values listed below the bars. Cs; cells). Error bars represent s.e.m. Yellow bars are mean values calculated for all kinetochores pooled from all cells in that class (n values listed below the bars, Ks; kinetochores). Triple asterisks denote differences with p < 0.005 (two-tailed Student’s test) for both blue vs. blue and yellow vs. yellow bars.