Concentration-dependent effects of BDE-47 on mRNA expression of PTGS2, PTGES, and HPGD in HTR-8/SVneo cells. Cells received no treatment (non-treated control, NT), or were treated with solvent control (DMSO, 0.7% v/v) or BDE-47 (5, 10, 15 or 20 μM), and then mRNA expression of target genes was quantified by qRT-PCR. A) Time-course and concentration-dependent response of BDE-47 on PTGS2 mRNA expression after 4, 12, or 24 h. B) BDE-47 concentration-dependent effects on PTGES mRNA expression after 24 h. C) BDE-47 concentration-dependent effects on HPGD mRNA expression after 24 h. *P<0.05, significantly different compared to solvent control within each time point (A) or experiment (B and C).