BDE-47 effects on COX activity in HTR-8/SVneo cells. COX activity was inferred by quantification of PGE2 in the culture medium from cells stimulated with exogenous arachidonic acid after BDE-47 treatment. A) PGE2 concentrations in medium of cells that were non-treated (NT, control), or treated with solvent control (DMSO, 0.7% v/v), 15 μM BDE-47, or 20 μM BDE-47 for 24 h. B) PGE2 concentrations in medium of cells that were non-treated (NT, control), or treated with solvent control (DMSO, 0.7% v/v) or 20 μM BDE-47 for 24 h in the absence or presence of the nonspecific cyclooxygenase (COX) inhibitor indomethacin or the COX-2 specific inhibitor NS 398. *P<0.05, significantly different compared to solvent control with no (±)-α-tocopherol cotreatment. #P<0.05, significantly different from each other.