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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Thromb Res. 2015 Jun 20;136(3):606–612. doi: 10.1016/j.thromres.2015.06.019

Figure 2. Enhancement of GPIbα binding to collagen-bound pVWF.

Figure 2

(A) Wall shear stress-dependent translocation velocity (mean ± S.E.M. of 20 platelets per point) on pVWF. 5 mM EDTA were added to the washed platelet perfusate. Data are presented as mean ± S.E.M. (B) Force-dependent of lifetime of GPIbα bonds with the pVWF. Lifetimes (mean ± S.E.M. of >20 measurements per point) of these bonds were measured by the force-clamp assay at each force. For each above assay, results by two immobilization methods were compared: directly coated pVWF (- collagen) and collagen-bound pVWF (+ collagen). Statistical analysis at each matched shear stress and force was performed to derive the p values. * = p < 0.05; ** = p < 0.01, *** = p < 0.001, assessed by unpaired, two-tailed Student's t-test.