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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Toxicol In Vitro. 2015 May 21;29(7):1369–1376. doi: 10.1016/j.tiv.2015.05.008

Figure 2. Knockdown of GDF15 abrogates GDF15 induction in hyperoxia.

Figure 2

BEAS-2B (a,c,e) and HPMEC (b,d,f) cells exposed to room air (room air-5% CO2) and 24, 48, or 72 h of hyperoxia (95% O2-5% CO2). GDF15 siRNA or negative control siRNA were transfected into BEAS-2B and HPMEC cells. Expression of GDF-15 was measured at the mRNA (a-d) and protein level (e,f) by ELISA. Values are means ± SEM of 3 independent biological replicates. Significant differences between GDF15 siRNA and negative control siRNA groups are indicated by *P <0.05, **P<0.01 and ***P <0.001.