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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Curr Opin Chem Biol. 2015 Jun 6;27:18–23. doi: 10.1016/j.cbpa.2015.05.014

Figure 1. Spectroscopic changes utilized for fluorogen activation.

Figure 1

Binding of a dye to a protein domain can result in changes in quantum yield (top), excitation wavelength (middle) or extinction coefficient/excitation cross-section (bottom). Combinations of these properties will result in high activation ratios.