Table 3.
DNA Authentication of Six Glycyrrhiza Hybrids (Root Sticks)
| sample code | DNA markers
|
TGc | claimed G. species | identified G. hybridsd | origin | |||
|---|---|---|---|---|---|---|---|---|
| ITSa,b | rbcL | matK | psbA-trnH | |||||
| BC750 | I-3/I-2 = ADD (14%) | R-1 | M-1 | T-2 | TG7 (cp) or TG3 (cp) | uralensis | G. uralensis × G. glabra | China, Xinjiang |
| BC752 | I-2/I-3 = ADD (12%) | R-1 | M-2 | T-3 | TG5 (cp) | inflata | G. uralensis × G. inf lata | China, Xinjiang |
| BC758 | I-3/I-2 = ADD (13%) | R-1 | M-2 | T-3 | TG5 (cp) | “Gan-cao” | G. uralensis × G. inf lata | China |
| BC772a | I-2/I-3 = ADD (≥86%) | R-1 | M-2 | T-3 | TG5 (cp) | inflata | G. uralensis × G. inf lata | China, Xinjiang |
| BC782c | I-2/I-3 = ADD (14%) | R-1 | M-2 | T-3 | TG5 (cp) | inflata | G. uralensis × G. inf lata | unknown |
| BC782d | I-2/I-3 = ADD (7%) | R-2 | M-1 | T-1 | TG8 (cp) | inflata | G. uralensis × G. inflata | unknown |
a
The occurrence of both I-2 and I-3 sequences for the ITS DNA marker is called ADD genotype and corresponds to “nucleotide additivities” often observed in hybrids between two species.27
b
The ITS ADD genotype is given as a ratio, with the primary genotype highlighted in bold, and the average secondary peak height ratio is given in parentheses.
c
Total genotype (TG) determined by the composition in DNA markers.
d
Genotype identified from the chloroplast DNA and corresponding to the putative maternal species (highlighted in bold).