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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Nat Cell Biol. 2015 Aug 17;17(9):1099–1111. doi: 10.1038/ncb3217

Figure 2. C/EBPα deficient adipocytes are viable and have normal lipid accumulation in vitro.

Figure 2

a. Western-blots of C/EBPα in protein extracts of differentiated adipocytes from SVF of Adn-C/EBPαflox/flox mice or control littermates, treated with doxycycline for 3 days. These images are representative of two independent Western-blot experiments.

b. Oil red O staining on differentiated adipocytes from SVF of Adn-C/EBPαflox/flox mice or control littermates, treated with doxycycline for 3 days (left). Relative Oil Red O intensity was measured by ImageJ software (right). n = 3 wells of a six-well plate for each group. This experiment is representative of three independent experiments.

c, d. immunofluorescence stains for C/EBPα (red), DAPI (blue) and perilipin (green) (c) or C/EBPα (red), DAPI (blue) and BODIPY (green) (d) on differentiated adipocytes treated with doxycycline for 3 days. These images are representative of two independent experiments.