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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Nat Cell Biol. 2015 Aug 17;17(9):1099–1111. doi: 10.1038/ncb3217

Figure 4. Mice with inducible C/EBPα elimination in adipocytes have impaired glucose and lipid metabolism during high fat diet challenge.

Figure 4

a. Experimental design: Adn-C/EBPαflox/flox mice started doxycycline (dox) HFD treatment at10 weeks of age to generate HFD Adn-C/EBPα−/− mice.

b. Body weights of both groups during doxycycline HFD feeding. n = 6 male mice (HFD control group), n = 11 male mice (HFD Adn-C/EBPα−/− group). *, P = 0.02 at day 34; **, P <0.001 at day 38; **, P <0.001 at day 44, compared to HFD control group. Data represent the mean ± s.e.m. Student’s t-test. This experiment is representative of three independent experiments.

c. Magnetic resonance imaging analysis of both groups after 4 weeks of doxycycline HFD feeding. Red mass represents adipose tissue. These images are from a single experiment.

d. NMR body fat and lean content of both groups after 4 weeks of doxycycline HFD feeding. n = 11 male mice per group. Data represent the mean ± s.e.m. This data is from a single experiment.

e. H&E staining (left) in sWAT, eWAT and BAT and average adipocyte size (right) of sWAT and eWAT, as indicated, of both groups after 4 weeks of doxycycline HFD feeding. These images are representative of two independent experiments. For adipocyte sizes, n = 53 cells (HFD control, sWAT); 42 cells (HFD Adn-C/EBPα−/−, sWAT); 43 cells (HFD control, eWAT); 47 cells (HFD Adn-C/EBPα−/−, eWAT). All data represent the mean ± s.e.m. Student’s t-test. All from one image shown in the figure. This data is from a single experiment.

f. Immunofluorescence staining for perilipin (red) and DAPI (blue) in sWAT, eWAT and BAT, as indicated, of both groups after 4 weeks of doxycycline HFD feeding. This image is representative of two independent experiments.

g, h. GTT (f) and ITT (g) were performed during the 4th and 6th week of doxycycline HFD feeding on Adn-C/EBPα−/− mice and their control littermates, the bar graph represents the area under the curve. For GTT, n = 10 male mice (HFD control group), n = 8 male mice (HFD Adn-C/EBPα−/− group). For ITT, n = 7 male mice (HFD control group), n = 6 male mice (HFD Adn-C/EBPα−/− group). *, P = 0.047, 0.039 for GTT 30, 90 minutes; **, P = 0.001 for GTT 60 minutes; *, P = 0.01 for GTT AUC; *, P = 0.048 for ITT 15, 60 minutes, *, P = 0.04 for ITT AUC, compared to HFD control group. All data represent the mean ± s.e.m. Two-way ANOVA. These experiment are all representative of two independent experiments.