Table 3.
Major gene products of Mannheimia haemolytica phage 3927AP2
| CDS | Gene name | Size (aa)/MW(kDa)/pI | Motifsa | Function | % Identity (aa) to related Mu-like phagesb | |
|---|---|---|---|---|---|---|
| Su-Mu | phiMhaMu2 | |||||
| 1 | c | 242/26.8/5.2 | S24_LexA-like (cd06529) | Transcriptional regulatory protein | 46.1 | 100 |
| 2 | ner | 75/8.4/9.8 | HTH_Tnp_Mu_1 superfamily (cl15894) | DNA-binding protein | 41.6 | 100 |
| 3 | A | 645/73.1/9.3 | Mu-transpos_C (pfam09299) | Transposase | 63.7 | 99.1 |
| 4 | B | 293/32.2/8.6 | HTH_LacI (cd01392) | Mu-like prophage FluMu DNA transposition protein B | 54.6 | 100 |
| 14 | gemA | 141/16.1/9.9 | DUF1018 (pfam06252) | Mu-like prophage protein gp16 | 66 | 100 |
| 18 | mor | 141/16.0/7.5 | Mor superfamily (cl02360) | Mor transcription activator family protein | 70.9 | 100 |
| 19 | lys | 177/19.8/7.7 | Glyco_hydro_108 (pfam05838) and PG_binding_3 (pfam09374) | Hypothetical protein | 20 | 94.4 |
| 25 | DNA-binding | 166/18.4/6.1 | TM (1) | DNA-binding protein | 48.5 | 100 |
| 27 | terL | 541/62.3/6.2 | Terminase_6 (pfam03237) | Terminase, large subunit | 71.3 | 100 |
| 28 | porT | 541/59.9/5.2 | COG4383 (COG4383) | Portal protein | 84 | 100 |
| 29 | F | 434/50.1/9.3 | Phage_Mu_F (pfam04233) | Phage head morphogenesis protein | 73.6 | 100 |
| 30 | G | 138/15.3/5.8 | COG5005 (COG5005) | Mu-like prophage FluMu G protein 2 | 73.9 | 100 |
| 31 | I | 356/38.9/5.2 | Mu-like_Pro (pfam10123) | Bacteriophage Mu I protein Gp32 | 76.9 | 99.7 |
| 32 | T | 305/33.9/5.4 | Mu-like_gpT (pfam10124) | Major head subunit T | 75.6 | 100 |
| 34 | J/K | 141/15.9/4.7 | DUF1320 (pfam07030) | Mu-like prophage protein gp36 | 71.5 | 100 |
| 35 | J/K | 213/23.6/5.9 | DUF1834 (pfam08873) | Mu-like prophage protein gp37 | 69.5 | 100 |
| 37 | L | 469/50.7/5.7 | Phage_sheath_1 (pfam04984) | Mu-like prophage FluMu tail sheath protein | 74.1 | 85.1 |
| 38 | M/Y | 124/13.7/4.8 | Tail_tube (pfam10618) | Phage tail tube protein | 75.8 | NA |
| 41 | tmp | 759/81.5/9.2 | PhageMin_Tail (pfam10145) | Tail length tape measure protein | 64.2 | 100 |
| 42 | N | 430/48.6/5.4 | DNA_circ_N (pfam07157) | Mu-like DNA circularization protein | 45.8 | 100 |
| 43 | P | 375/41.8/7.6 | Phage_GPD superfamily (cl15796) | Mu-like tail protein P | 75 | 99.7 |
| 44 | Q | 219/22.8/6.9 | Phage_Mu_Gp45 (pfam06890) | Mu-like baseplate assembly protein | 70.8 | 99.5 |
| 45 | V | 116/13.1/6.8 | GP46 (pfam07409) | Mu-like protein gp46 | 81.9 | 100 |
| 46 | W | 353/38.7/4.7 | Baseplate_J superfamily (cl01294) | Mu-like baseplate J protein | 54.1 | 100 |
| 47 | R | 196/21.9/5.2 | DUF2313 (pfam10076) | Tail protein | 35.8 | 99.5 |
| 48 | S | 686/75.7/5.9 | Pectate_lyase_3 (pfam12708) | Mu-like prophage FluMu defective tail fiber protein | 20.6 | NA |
aTM: transmembrane α-helice
bIdentity of amino acids sequence was calculated by ALIGN [66]