Table 1.
Ion transitions and instrumental parameters for their LC–MS/MS quantification in MRM mode
| Sample | Ion mode | MRM | CV | CE | LOQ | LOD |
|---|---|---|---|---|---|---|
| Benzoylmesaconine | + | 590.33 > 77.02 | 52 | 78 | 2.1 | 0.73 |
| Aconitine | + | 646.39 > 586.34 | 50 | 34 | 1.9 | 0.6 |
| Mesantine | + | 632.33 > 105.04 | 42 | 56 | 2.2 | 0.9 |
| Benzoylhypacoitine | + | 574.32 > 542.35 | 46 | 34 | 1.8 | 0.6 |
| Benzoylaconitine | + | 604.41 > 105.04 | 58 | 54 | 4.3 | 1.7 |
| Hypaconitine | + | 616.36 > 556.31 | 42 | 32 | 1.9 | 0.61 |
| Reserpine(is) | + | 609.33 > 174.07 | 40 | 42 | – | – |
IS internal standard, CE collision energy (eV), CV cone voltage (V), MRM specific mass transition, LOQ limit of quantification (ng/ml), LOD limit of detection (ng/ml)