. Author manuscript; available in PMC: 2016 Aug 1.

Published in final edited form as: J Mol Endocrinol. 2015 Jul 28;55(2):107–117. doi: 10.1530/JME-15-0116

Table 1.

Forward primer sequences used for site-directed mutagenesis studies of hMC3R. The mutated codons are underlined.

Constructs	Primer sequences
D332A	CAACTCCGTCATCGCCCCACTCATCTACG
P333A	CTCCGTCATCGACGCACTCATCTACGC
L334A	CTCCGTCATCGACCCAGCCATCTACGCTTTCCGG
I335A	CATCGACCCACTCGCCTACGCTTTCC
Y336A	CATCGACCCACTCATCGCCGCTTTCCGGAGCCTG
A337G	CCACTCATCTACGGTTTCCGGAGCCTG
F338A	CCCACTCATCTACGCTGCCCGGAGCCTGGAATTG
R339A	CTCATCTACGCTTTCGCGAGCCTGGAATTGCG
S340A	CATCTACGCTTTCCGGGCCCTGGAATTGCGCAAC
L341A	CTACGCTTTCCGGAGCGCGGAATTGCGCAACACC
E342A	CGCTTTCCGGAGCCTGGCATTGCGCAACACCTTTAG
L343A	CTTTCCGGAGCCTGGAAGCGCGCAACACCTTTAGGG
R344A	CGGAGCCTGGAATTGGCCAACACCTTTAGGGAG
N345A	GGAGCCTGGAATTGCGCGCCACCTTTAGGGAGATTC
T346A	CTGGAATTGCGCAACGCCTTTAGGGAGATTC
F347A	GAATTGCGCAACACCGCTAGGGAGATTCTCTG
R348A	GCGCAACACCTTTGCGGAGATTCTCTGTG
E349A	CAACACCTTTAGGGCGATTCTCTGTGGC
I350A	CACCTTTAGGGAGGCTCTCTGTGGCTGC
L351A	CACCTTTAGGGAGATTGCCTGTGGCTGCAACGGC