Figure 1. Effect of T-bet on Th cell proliferation. CD4⁺ Th cells were isolated from WT and T-bet KO mice in the absence (-, A) or presence (+, B) of exogenous rhIL-2 and stimulated with different amounts of anti-CD3 under non-skewing (NS) conditions for 3 days. Cells were seubsequently incubated with 3H-thymidine and subjected to liquid scintillation counting using Micro Beta and TopCount equipment (Perkin Elmer). Y-axes represent count per minute (CPM). Three independent experiments were performed using 6 mice for each experiment. Data are expressed as the average ±SD of 6 mice. ^*p<0.05, ^**p<0.005.