Figure 6. Suppression of cell proliferation by T-bet. Stable T-bet expressing cell clones were established in EcR-HEK cells and maintained in DMEM containing G418 and Zeocin. (A) Stable cells were treated with PonA for 24 h. Protein extracts were prepared from each stable cell clone and analyzed by immunoblotting with the T-bet (4B10) Ab. (B) Stable cell clones were cultured with or without PonA at the indicated concentrations. Cells were counted by Trypan blue exclusion assay every 2 days in triplicate. Data are expressed as the average±SD from 3 separate experiments. ns, not significant; ^*p<0.05; ^**p<0.005; ^***p<0.0005.