Table 1.
Primers for expressed genes in WT and trk-gene knockout mutant strains of Mtb.
| Gene name | Forward primer (bp) | Reverse primer (bp) | Fragment lengtha | Tmb |
|---|---|---|---|---|
| ceoB c | CGG CGA CAA CTC CAA CAT (18) | CGG CAC ACC GAA GGT TTC (18) | 58 | 86.2 |
| ceoC c | CTG CTG GAG TCG ATT CAC CT (20) | ACC GCG AAT TCG GTC TTG (20) | 107 | 88.9 |
|
| ||||
| kdpA | GAT TGA ACG GCC TAC TGG TC (20) | CTG GAT CTT CTT GCC GAG AT (20) | 95 | 87.5 |
| kdpB | CTG GGC TGA CGA TCA TCT TT (20) | CGT GGT CGG AAT GAG ACA C (19) | 122 | 90.6 |
| kdpC | TAC CGC AAG GAA AAC AAT CTG (21) | GGC ATT GAC CAC CGA TATG (19) | 102 | 90.2 |
| kdpF | AAC ATC GTC GGG TTG GTG (18) | GCG AAT AGG AAC GCC ATT AG (20) | 50 | 83.7 |
|
| ||||
| kdpD | AGT CCA TCG ACC AAC TCA CC (20) | CAC CGC TTC CTC CAG GTA T (19) | 110 | 91.4 |
| kdpE | TGG AAT GGA CGA GTT TCT GG (20) | GAC GGT GAA TGA ATC GGT TT (20) | 103 | 91.4 |
|
| ||||
| sigA | CAA GGA CGC CGA ACT CAC (18) | CTT GCC GAT CTG TTT GAG GT (20) | 64 | 87.4 |
aSize of the amplicon synthesized by RT-PCR in base pair (bp).
bMelting temperature derived from melting curve.
cGene encoding Trk system.