Table 3.
Four mutations with significant differences in mutant allele frequency between the three panels.
| Cell line | Gene | Transcript | Variant | Fluidigm custom panel |
SureSeq Solid Tumour panel | Ion AmpliSeq Cancer Hotspot Panel | Sanger sequencing zygosity status |
|---|---|---|---|---|---|---|---|
| 97-7 | FGFR3 | NM_00142.4 | c.746C>G | N/A | 81.4% | 34.6% | Heterozygous |
| KU-19-19 | NRAS | NM_002524.3 | c.182A>G | 48.2% | 50.5% | 86.3% | Heterozygous |
| J82 | TP53 | NM_000546.5 | c.960G>C | 85.7% | 51.3% | N/A | Heterozygous |
| LUCC3 | TP53 | NM_000546.5 | c.840_841insAA | 57.7% | 58.9% | 96.4% | Heterozygous |