Fig. 5. IFN-γ, IL-4, and IL-5 expression levels. Messenger RNA levels of IFN-γ (A), IL-4 (C), and IL-5 (E) were measured by quantitative reverse transcription-polymerase chain reaction. Protein levels of IFN-γ (B), IL-4 (D), and IL-5 (F) were measured by enzyme-linked immunosorbent assay. Levels of IL-4 and IL-5 were inhibited in OVA+TSA group compared with OVA group. However, IFN-γ level did not increase in OVA group. IFN-γ, interferon-gamma; IL, interleukin; OVA, ovalbumin; TSA, trichostatin A. ^*P<0.05 compared to controls. ^†P<0.05 compared to OVA.