Fig. 2. SIRT1 deletion increases NF-κB acetylation and induces NF-κB nuclear translocation in HFD-fed mice. Western blots of SIRT1 (A) and acetylated NF-κB p65 (B) in liver homogenates from WT and KO mice fed ND or HFD. (C, D) Western blots of cytosolic and nuclear NF-κB and the nuclear/cytosolic (N/C) ratio of NF-κB levels. Band intensity was normalized to β-actin or α-tubulin. Data are presented as mean±SEM. ^*p<0.05 for WH mice versus WN mice. ^†p<0.05 for KH mice versus KN mice. ^#p<0.05 for KH mice versus WH mice. WN, ND-fed WT mice; WH, HFD-fed WT mice; KN, ND-fed KO mice; KH, HFD-fed KO mice.