Fig. 4. SIRT1 deletion aggravates hepatic steatosis in HFD-fed mice. (A) Histological analysis of hepatic fat accumulation by H&E and Oil Red O staining. Scale bar=100 µm. (B) Western blots of nuclear SREBP1 in liver homogenates from WT and KO mice fed an ND or HFD. Band intensity was normalized to nuclear p84 protein. (C) Hepatic TG levels in supernatant fractions of liver homogenates. Data are presented as mean±SEM. ^*p<0.05 for WH mice versus WN mice. ^†p<0.05 for KH mice versus KN mice. ^#p<0.05 for KH mice versus WH mice. WN, ND-fed WT mice; WH, HFD-fed WT mice; KN, ND-fed KO mice; KH, HFD-fed KO mice.