Fig. 5. SIRT1 deletion increases hepatic CTGF and α-SMA expression in HFD-fed mice. (A) Western blots of CTGF in liver homogenates from WT and KO mice fed ND or HFD. (B) Immunohistochemistry for CTGF detection in liver sections. (C) Western blots of α-SMA in liver homogenates from WT and KO mice fed ND or HFD. (D) Immunohistochemistry for α-SMA detection in liver sections. Band intensity was normalized to β-actin. Data are presented as mean±SEM. ^*p<0.05 for WH mice versus WN mice. ^†p<0.05 for KH mice versus KN mice. ^#p<0.05 for KH mice versus WH mice. WN, ND-fed WT mice; WH, HFD-fed WT mice; KN, ND-fed KO mice; KH, HFD-fed KO mice. Scale bar=100 µm.