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. 2015 Aug 31;6:664. doi: 10.3389/fpls.2015.00664

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Copyright © 2015 Shimojima, Madoka, Fujiwara, Murakawa, Yoshitake, Ikeda, Koizumi, Endo, Ozaki and Ohta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Growth phenotypes and TAG accumulation in shoots of WT Arabidopsis plants grown under Pi- or N-depleted conditions. WT plants (10 d old) were transferred to MS agar containing 1% (w/v) sucrose with (+Pi; 1 mM) or without (−Pi; 0 mM) Pi for 10 d, or with (+N; 3.5 mM) or without (−N; 0 mM) N for 7 d. (A) Growth under Pi-depleted or N-depleted conditions. Bars = 1 cm. (B) TAG levels in shoots under Pi-depleted or N-depleted conditions. DW, dry weight. (C) Shoot fresh weight under Pi-depleted or N-depleted conditions. B, before transfer to Pi- or N-depleted conditions. (D) TAG content in shoots per seedling under N- or Pi-depleted conditions. Data are the means ± SD from three independent experiments.