Table 2.
NanoESI-LC-MS/MS based identification of proteins from 2D gel, spot 1, after POD activity staining (Data analysis with LabKey).
| Hit | Protein group | Inference class | Description | Sequence coverage [%] | #Validated peptides | #Validated spectra | Theoretical MW [kDa] |
|---|---|---|---|---|---|---|---|
| 1 | B6TPH0, C0PK05 | Unrelated proteins | Lactoylglutathione lyase | 21.3 | 10 | 22 | 35.1 |
| 2 | B6UH67 | Single protein | Late embryogenesis abundant protein D-34 | 41.7 | 7 | 11 | 27.2 |
| 3 | B6SGN7 | Single protein | Embryonic protein DC-8 | 16.1 | 6 | 12 | 32.3 |
| 4 | B4FFK9 | Single protein | Uncharacterized protein/Lipoprotein1/ Secreted protein1 | 19.0 | 5 | 6 | 27.7 |
| 5 | B6TK66 | Single protein | Embryonic protein DC-8 | 10.3 | 2 | 2 | 33.6 |
| 6 | K7VEA3, K7VM99 | Isoforms | Uncharacterized protein/ Late embryogenesis abundant protein1 | 12.5 | 2 | 2 | 28.4 |
1Evaluated by BLASTP.