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. 2012 Feb 10;3(1):141–160. doi: 10.3390/insects3010141

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© 2012 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Phylogenetic analysis of ALB-derived and O’Donnell Fusarium solani isolates using the translation elongation factor 1-alpha locus. A rooted maximum likelihood tree was generated using TEF1-α sequences from all cultured representatives and a single representative from each OTU detected through culture-independent methods (n = 500 bootstrap replicates). These sequences were placed within the F. solani species complex, utilizing the dataset of O’Donnell [44]. Mating populations (abbreviated MP) are listed based off of the O’Donnell dataset. Nodes with bootstrap support values > 50 are reported, ML distance scale bar = 10 changes. ALB-derived isolates formed 3 strongly supported clusters, denoted as OTU1, OTU2A, and OTU2B. A single NYC isolate and all culture-independent sequences generated from this population were grouped into OTU1, while all MA-derived isolates were classified into cluster 2b. PSU-derived isolates can be observed in both OTU1 and OTU2a.