Fig. 4. Deferoxamine induced HIF-1α in a dose-dependent manner in human small airway epithelial cells (HSAEpCs) (A). HSAEpCs were incubated with 10, 20, 30 or 65 µM DFX for 8 hr, and HIF-1α expression was evaluated by Western blotting. HSAEpCs were incubated with 65 µM DFX for the indicated times (B). The expression of HIF-1α was analyzed by Western blotting. DFX, deferoxamine; HIF, hypoxia-inducible factor.