Table S3.
Effect of 3-nitrooxypropanol on fatty acid composition of milk fat (g/100 g of total fatty acids) in dairy cows
| Diet* | P value† | |||||||
| Fatty acid | Control | High3NP | Low3NP | Medium3NP | SEM‡ | C vs. Trt. | L | Q |
| 4:0 | 4.10 | 4.17 | 4.13 | 4.03 | 0.083 | 0.96 | 0.60 | 0.27 |
| 6:0 | 2.12 | 2.32 | 2.23 | 2.21 | 0.041 | <0.01 | 0.11 | <0.01 |
| 8:0 | 1.21 | 1.40 | 1.34 | 1.33 | 0.028 | <0.001 | <0.01 | <0.01 |
| 10:0 | 2.82 | 3.35 | 3.20 | 3.21 | 0.083 | <0.001 | <0.01 | <0.01 |
| 12:0 | 3.28 | 3.89 | 3.72 | 3.79 | 0.112 | <0.001 | <0.01 | 0.03 |
| 14:0 | 10.1 | 10.2 | 10.3 | 10.3 | 0.14 | 0.18 | 0.17 | 0.79 |
| 14:1 | 0.90 | 0.88 | 0.90 | 1.04 | 0.051 | 0.58 | 0.12 | 0.06 |
| 15:0 | 0.93 | 1.24 | 1.08 | 1.10 | 0.059 | <0.01 | 0.07 | <0.01 |
| 16:0 | 25.6 | 26.0 | 24.9 | 25.7 | 0.43 | 0.87 | 0.66 | 0.97 |
| 16:1 | 1.33 | 1.31 | 1.20 | 1.29 | 0.07 | 0.40 | 0.40 | 0.63 |
| 17:0 | 0.47 | 0.52 | 0.49 | 0.49 | 0.013 | 0.03 | 0.31 | 0.03 |
| 18:0 | 10.2 | 10.1 | 10.2 | 9.41 | 0.414 | 0.48 | 0.25 | 0.38 |
| 18:1, trans-4 | 0.031 | 0.030 | 0.030 | 0.028 | 0.0011 | 0.19 | 0.08 | 0.44 |
| 18:1, trans-5 | 0.029 | 0.034 | 0.026 | 0.037 | 0.0072 | 0.66 | 0.62 | 0.78 |
| 18:1, trans-6–8 | 0.45 | 0.40 | 0.43 | 0.41 | 0.013 | 0.04 | 0.11 | 0.28 |
| 18:1, trans-9 | 0.42 | 0.35 | 0.35 | 0.35 | 0.037 | 0.09 | 0.15 | 0.42 |
| 18:1, trans-10 | 0.84 | 0.72 | 0.81 | 0.77 | 0.069 | 0.36 | 0.56 | 0.54 |
| 18:1, trans-11 | 1.17 | 0.98 | 1.14 | 0.93 | 0.060 | 0.03 | 0.03 | 0.95 |
| 18:1, trans-12 | 1.35 | 0.94 | 0.60 | 1.32 | 0.555 | 0.53 | 0.75 | 0.37 |
| 18:1, cis-9 | 23.6 | 22.1 | 23.5 | 22.8 | 0.758 | 0.35 | 0.59 | 0.49 |
| 18:1, cis-11 | 1.48 | 1.38 | 1.53 | 1.51 | 0.043 | 0.81 | 0.41 | 0.12 |
| 18:2, cis-9,cis-12 | 4.23 | 4.03 | 4.25 | 4.31 | 0.080 | 0.73 | 0.38 | 0.04 |
| 20:0 | 0.10 | 0.11 | 0.11 | 0.10 | 0.004 | 0.81 | 0.31 | 0.13 |
| 18:3 | 0.46 | 0.45 | 0.47 | 0.47 | 0.007 | 0.80 | 0.35 | 0.36 |
| CLA-cis-9,trans-11 | 0.55 | 0.43 | 0.51 | 0.46 | 0.021 | <0.01 | 0.02 | 0.06 |
| CLA-trans-10,cis-12 | 0.005 | <0.001 | <0.001 | 0.001 | 0.0014 | 0.01 | 0.04 | 0.16 |
| Others | 2.46 | 2.52 | 2.57 | 2.59 | 0.062 | 0.17 | 0.12 | 0.98 |
| Total trans fatty acids | 4.20 | 3.51 | 3.35 | 3.92 | 0.602 | 0.37 | 0.58 | 0.35 |
| ΣSFA | 60.9 | 63.3 | 61.7 | 61.7 | 0.52 | 0.02 | 0.35 | <0.01 |
| ΣMUFA | 29.2 | 27.1 | 28.3 | 28.2 | 0.51 | 0.02 | 0.19 | 0.03 |
| ΣPUFA | 5.23 | 4.91 | 5.23 | 5.25 | 0.088 | 0.34 | 0.64 | 0.02 |
CLA, conjugated linoleic acid; SFA, saturated fatty acids, MUFA, monounsaturated fatty acids; PUFA, poly-unsaturated fatty acids.
*
Control = 0 mg/kg of 3NOP, Low3NOP = 40 mg/kg of 3NOP, Medium3NOP = 60 mg/kg 3NOP, and High3NOP = 80 mg/kg 3NOP (dietary dry matter basis). Data are presented as least squares means.
†
Contrasts: C vs. Trt., Control vs. all 3NOP treatments; L, linear effect of 3NOP; Q, quadratic effect of 3NOP.
‡
n = 140 (n represents number of observations used in the statistical analysis).