Fig. 3.

MZ MΦ and B-cell activation are increased in lupus-prone mice lacking myeloid GCN2 function. (A) Splenocyte numbers from 6-mo-old mice of the indicated genotype. (B) Representative Western blot of whole spleen lysate from two mice per group described in A. m, mouse. (C–E) Flow cytometry analysis of groups described in A. In D, B-cell plots shown are gated on a B220⁺ population. Graphs in E are gated on the markers indicated above. Bars represent mean ± SD values for eight mice. *P < 0.05, **P < 0.01, Student’s t test. ns, not significant. Experiments were repeated four times, with similar results.