Table S2.
Primer | Sequence (5′-3′) |
pir-1 | GAGTCGACGGCATCAGTTACGTTAG |
pir-2 | GAGGTACCCGATGAATTTGGTACAG |
pir-3 | GTGGTACCTCGACAGTCCAATCGTGAG |
pir-4 | GAGGTACCGTCAGAGAACCACGGTTG |
pir-5 | GAGAGCTCCGTTCACAGTCGCTACAG |
pir-8 | CTGAGCTCCCTTAGTGCCAGCGATC |
pnd-1 | GCCGATACGGTGAATGAATC |
pnd-2 | GCATCGCTTGCCTCCAGTC |
pnd-3 | CAGGAAGGGCGGTTACAG |
pnd-4 | GTCATAGTATGGCGATGAC |
PirA_1F | ATGAGTAACAATATAAAACATG |
PirA_336R | TTAGTGGTAATAGATTGTACAG |
PirA_1F_NdeI | GCGCATATGAGTAACAATATAAAACATG |
PirA_336R_XhoI | GCGCTCGAGGTGGTAATAGATTGTACAG |
PirB_1F | ATGACTAACGAATACGTTGTAAC |
PirB_1317R | CTACTTTTCTGTACCAAATTCAT |
PirB_1F_NdeI | GCGCATATGACTAACGAATACGTTGTAAC |
PirB_1317R_XhoI | GCGCTCGAGCTTTTCTGTACCAAATTCAT |
PirB400F | GAGCCAGATATTGAAAACATTTGG |
PirB400R | CCACGCAGCGAGTTCTGTAATGTA |
VP tdh F | GTAAAGGTCTCTGACTTTTGGAC |
VP tdh R | TGGAATAGAACCTTCATCTTCACC |
VP trh F | TTGGCTTCGATATTTTCAGTATCT |
VP trh R | CATAACAAACATATGCCCATTTCCG |
VP tlh F | AAAGCGGATTATGCAGAAGCACTG |
VP tlh R | GCTACTTTCTAGCATTTTCTCTGC |
VP ldh F | GTTCTTCGCCAGTTTTGCGT |
VP ldh R | GACATTACGTTCTTCGCCGC |
AP1F | CCTTGGGTGTGCTTAGAGGATG |
AP1R | GCAAACTATCGCGCAGAACACC |
AP2F | TCACCCGAATGCTCGCTTGTGG |
AP2R | CGTCGCTACTGTCTAGCTGAAG |
pnd-9 | GCCTCTTCACTGACTGGA |
pnd-10 | GAGCGGCTTCCTGTAACC |
AP1-F16 | GTAGTAAAGAGGACTTGAGGA |
AP2-R1 | ACACTAAGTCTTGCCGTTTC |
The restriction enzyme cutting sites used for cloning purpose are underlined.