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. 2015 Aug 10;112(34):E4726–E4734. doi: 10.1073/pnas.1514105112

Fig. 1.

Fig. 1.

Validation of splicing targets of mutSRSF2. (A, Lower Left) P95H allele expression in the mutant CRISPR clones. (Lower Right) Expression of SRSF2 in 293T cells either mock transfected (–) or transfected with plasmid encoding HA-tagged WT (W) or P95H mutSRSF2 (P). In the immunoblot analysis, the mAb104 antibody was used to detect endogenous and HA-tagged SRSF2. (Upper) RT-PCR products of exon inclusion and exclusion isoforms of ATF2. (B–G) RT-PCR products of splicing isoforms of MELK (B), PFKM (C), CDK5RAP2 (D), ARMC10 (E), DGUOK (F), and WDR45 (G). In all panels, each CRISPR clone was considered as one independent experiment. Because we had four WT and four mutSRSF2 CRISPR clones, n was 4 (as indicated). For 293T cells, three independent transfection experiments were performed (n = 3). Rounded percentages of exon inclusion or exclusion and SDs are shown as indicated. *P < 0.05, **P < 0.01, and ***P < 0.001.