Fig. 6.

Interactions of SRSF2 with U2AF1, snRNP70, and SF3B1 are unaffected by the P95H mutation. Coimmunoprecipitation was performed, in the presence of RNase, with anti-HA rabbit polyclonal antibody using extracts of 293T cells either mock-transfected (–) or transfected with plasmid expressing HA-tagged eGFP (G), WT (W), or P95H mutSRSF2 (P). The heavy and light chains of anti-HA polyclonal antibody were detected in the anti-ACTIN (rabbit) and anti-U2AF1 (rabbit) immunoblots due to the use of the anti-rabbit secondary antibody. The mAb104 antibody was used to detect endogenous and HA-tagged SRSF2 proteins.