Fig. S6.

Neutralization of breakthrough T/F Envs by prechallenge serum from vaccinated protected monkeys compared with the autologous prechallenge serum. Neutralization of each breakthrough T/F Env by prechallenge serum samples from monkeys that were immunized with the same vaccine but remained uninfected after 12 challenges is shown in the individual panels. The autologous serum sample is represented by a red (DDMM), green (MMM), purple (D_40LD_40LMM), or blue (DgDgMM) line in each graph; the vaccination group-matched serum samples from uninfected animals against the same T/F Env are shown in gray. The reciprocal of the serum dilution is plotted on the x axis on a log10 scale, and the percent of viral infectivity is plotted along the y axis relative to the virus-only control at 100%. Each serum–Env combination was run in duplicate, and each assay was repeated independently at least twice. The variation for each point in the infectivity curve within each group is indicated by the error bars, which represent the SEM.