Skip to main content
. 2015 Aug 10;112(34):10786–10791. doi: 10.1073/pnas.1422749112

Fig. 2.

Fig. 2.

Antileukemic in vivo effect on MA9Ras cells depends on murine effector cells. (A) Fold expansion of MA9Ras cells in suspension cultures with addition of 10 μg/mL IL1RAP-targeting antibodies mAb81.2 and mAb3F8 or an isotype control antibody (means ± SEM from four experiments with technical duplicates). (B) Relative levels of viable, apoptotic, and necrotic MA9Ras cells after treatment with 10 μg/mL mAb81.2, mAb3F8, or an isotype control antibody. Staurosporine treatment (STP) was used as positive control for apoptosis (means ± SEM from three experiments with technical duplicates). (C and D) NSG mice were engrafted with MA9Ras cells and treated with mAb81.2 or an isotype control antibody (n = 5 in both groups). Graphs show BM (C) and spleen (D) leukemic cell frequency at death 39 d after transplantation (means ± SD). (E) IL-1 activates NF-kβ in the HEKblue IL1R1 reporter cell line. The graph shows the NF-kβ activation measured by absorbance in the presence of IL-1 upon addition of mAb81.2 or mAb3F8 or an isotype control antibody (means ± SEM from technical duplicates; one representative experiment out of three). (F) Absorbance of NF-kβ activation in the HEKblue IL1R1 reporter cell assay in the absence of IL-1 with IL1RA included as a control (one representative experiment out of three). P values were calculated with Student’s t test.