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Complete mutagenesis of traptamer LI-5. C127 cells were infected with low-titer retrovirus stocks of MSCV vector or MSCV expressing BPV E5, wild-type LI-5, and a series of LI-5 mutants in which the wild-type amino acid at each position was changed to either leucine or isoleucine, as appropriate. Plates were stained with Giemsa 3 wk later. These plates were assembled from several different assays and not normalized for virus titer, but are representative of normalized activity.
