Table S3.

Oligonucleotides used in this study

Primer	5′ – 3′ sequence
1	TTAACCCGGGCGCGCGGCACC
2	GAGTGAGCTCTCAGCGTTTCACCAATC
3	GCGCCCCGGGAACAGGAAAACGAAAGAGATC
4	GCGCGAGCTCTCATTTCGTTCTCCTTCC
5	CCGGTGTTCTACTGGGTGCGTGCCGAGAAC
6	GTTCTCGGCACGCACCCAGTAGAACACCGG
7	CCGGTGTTCTACCGTGTGCGTGCCGAGAAC
8	GTTCTCGGCACGCACACGGTAGAACACCGG
9	CAAGCTCGGCGAACGTGCGCGAGTC
10	GACTCGCGCACGTTCGCCGAGCTTG
11	CCTCAAGCTCGGCGAATGGGCGCGAGTCGAC
12	GTCGACTCGCGCCCATTCGCCGAGCTTGAGG
13	GGGCAAGGAAATGGAAGACCTGCGCTACAC
14	GTGTAGCGCAGGTCTTCCATTTCCTTGCCC
15	GAACAAGGGCAAGGAAATGTCTGACCTGCGCTACACCTG
16	CAGGTGTAGCGCAGGTCAGACATTTCCTTGCCCTTGTTC
17	GCTACACCTGGGACCGCGGCCAGC
18	GCTGGCCGCGGTCCCAGGTGTAGC
19	CTGCGCTACACCTGGTCTCGCGGCCAGCGCG
20	CGCGCTGGCCGCGAGACCAGGTGTAGCGCAG
21	GCGCTACACCTGGAACGAAGGCCAGCGCGACG
22	CGTCGCGCTGGCCTTCGTTCCAGGTGTAGCGC
23	GCGCTACACCTGGAACTCTGGCCAGCGCGACG
24	CGTCGCGCTGGCCAGAGTTCCAGGTGTAGCGC
25	GGAACCGCGGCCAGTCTGACGGCCTGGCC
26	GGCCAGGCCGTCAGACTGGCCGCGGTTCC
27	CAGGGGCTGCGACGCGCTAAGGACAAGTTCCAG
28	CTGGAACTTGTCCTTAGCGCGTCGCAGCCCCTG
29	CAGGGGCTGCGACGCCTGAAGGACAAGTTCCAG
30	CTGGAACTTGTCCTTCAGGCGTCGCAGCCCCTG
31	GCTGCGCTTCGACCTGGAGAACGCTGGCGCTGCTATGAAAGACCTGCGCTACACCTG
32	CCAGGTGTAGCGCAGGTCTTTCATAGCAGCGCCAGCGTTCTCCAGGTCGAAGCGCAC
33	AATTCTAATACGACTCACTATAGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAG
34	GATCCTGGTGGAGCCTAGCGGGATCGAACCGCTGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTAAGGCCCCTATAGTGAGTCGTATTAG
35	AATTCTAATACGACTCACTATAGGGTCGTTAGCTCAGTCGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGATCCACCAG
36	GATCCTGGTGGATCGTGTAGGATTCGAACCTACGACCAATTGGTTAAAAGCCAACTGCTCTACCGACTGAGCTAACGACCCTATAGTGAGTCGTATTAG
37	AATTCTAATACGACTCACTATAGGGTCGTTAGCTCAGTCGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCAG
38	GATCCTGGTGGGTCGTGTAGGATTCGAACCTACGACCAATTGGTTAAAAGCCAACTGCTCTACCGACTGAGCTAACGACCCTATAGTGAGTCGTATTAG

Restriction sites are shown in italics. Bold fonts indicate exchanged nucleotides for mutagenesis of the catalytic domain of A-PGS from P. aeruginosa.