Fig. 3.
Bioinformatics analysis of differentially expressed proteins in Fmr1 KO mice. (A) Pie chart shows the gene ontology of changed proteins in the category of cellular localization (Upper) and molecular function (Lower); the components in each category were displayed as a percentage of proteins. (B) Functional enrichment analysis of changed proteins by Fisher’s exact test. The top 12 most significant functional categories were plotted with the x axis representing significance values. (C) Representative protein complexes identified in the CORUM database from the significantly altered proteins in PND17. Red color indicates significantly up-regulated proteins in KO mice. (D) Comparison of the genes from all quantified proteins and significantly changed proteins to gene entries from SFARI autism database, gene-to-cognition database (G2Cdb:PSP), as well as from FMRP target dataset by Darnell et al. (18). Proteins listed in the green oval are autism-associated gene products that are up-regulated in KO mice and intersect with all three data sets. The P value represents the enrichment odds ratio of the overlap as determined by χ2 test.