Skip to main content
. 2015 Aug 26;56(9):5656–5669. doi: 10.1167/iovs.15-17204

Figure 3.

Figure 3

Involvement of SMAD3 signaling in TGF-β2-induced expression of Hic-5 in HTM cells. Serum-starved HTM cells were treated with TGF-β2 (8 ng/mL for 14 hours) alone or in the presence of SMAD3 inhibitor-SIS3 (10 μM for 12 hours) and examined for changes in levels of Hic-5 by immunoblotting analysis. β-actin was immunoblotted as a loading control. While stimulation with TGF-β2 resulted in an increase in Hic-5 protein levels, treatment with SIS3 either alone or in the presence of TGF-β2 led to significant reduction in the levels of Hic-5 to below those of untreated controls (n = 4, mean ± SD). *P < 0.05.