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. 2015 Aug 26;56(9):5656–5669. doi: 10.1167/iovs.15-17204

Figure 4.

Figure 4

Hic-5 induces formation of actin stress fibers and FAs in HTM cells. (A) Serum-starved HTM cells infected with adenovirus expressing either Hic-5 or DsRed and immunoblotted using an anti-Hic-5 antibody exhibit a significant increase in Hic-5 protein levels compared to DsRed controls (n = 4, mean ± SD). *P < 0.05. β-actin was immunoblotted as a loading control. (B, C) Expression of recombinant Hic-5 induces formation of actin stress fibers and a contractile morphology in HTM cells, with Hic-5 distributing to the leading ends of actin stress fibers and along the actin stress fibers (arrows). Additionally, Hic-5 colocalizes with vinculin to the FAs (arrows) in HTM cells overexpressing Hic-5 compared with DsRed-expressing cells. Scale bar: 50 μm.