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. 2015 May 9;107(8):djv123. doi: 10.1093/jnci/djv123

Figure 3.

Figure 3.

Effect of PF-04554878 on phosphorylation of focal adhesion kinase (FAK), AKT, and ERK. A) Immunoblot analysis of phosphorylated (Y397) and total FAK protein levels after one hour of treatment with increasing doses of PF-04554878 in human pancreatic neuroendocrine tumor cell lines. B) Effect of one hour or (C) three hours of PF-04554878 treatment on FAK, AKT, and ERK1/2 activation as determined by immunoblot analysis of phosphorylated FAK (Y397), AKT (S473), and ERK1/2 (Thr202/204) in comparison with total protein levels. Tubulin or GAPDH were used as loading controls. *Loading control for phosphoproteins. †Loading control for total proteins. FAK = focal adhesion kinase.