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. 2015 Aug 20;74(9):862–866. doi: 10.1097/NEN.0000000000000234

FIGURE 3.

FIGURE 3

Double staining of donor-derived cells by immunohistochemistry. (A) Double staining of donor-derived cells with anti–HLA-A2 antibody (brown) and anti-CCR2 antibody (blue). Arrows indicate CCR2-negative and HLA-A2–positive cells; arrowheads indicate double-positive cells. (B) Migrated HLA-A2–positive cells were counted in 3 independent sections; the frequency of CCR2-positive cells and CCR2-negative cells is shown in the bar graph. Bars indicate mean ± SD. (C) Double staining of donor-derived cells with anti–HLA-A2 antibody (brown) and anti-CX3CR1 antibody (blue). Arrow indicates a double-positive cell indicating donor origin; arrowhead indicates a CX3CR1-positive and HLA-A2–negative cell indicating host origin.