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Photomicrograph depicting the use of fluorescent in situ hybridization (FISH) to evaluate the spatial distribution of the altered Schaedler flora (ASF) relative to the mucosal epithelium (DAPI stain - blue) of the proximal colon in a C3H/HeN mouse colonized with the ASF and Escherichia coli. The EUB338-FITC probe (green) was used as a nonspecific probe to detect the ASF with a species-specific Cy3-labeled probe for E. coli (orange).
