Figure 8.
Translation of WT and mutated X-OATs. The introduced mutations are shown with their designations (A). The M2 has both the M1 and M3 mutations. M1: X-OATR180T; M2: X-OATR180T/L402P; M3: X-OATL402P; M4: X-OATK292N; WT: X-OATWT. Synthetic WT and mutated X-OAT mRNA were translated in a TNT-coupled reticulocyte lysate system (Promega) with [35S] methionine. Products were analyzed by autoradiography on an SDS Tris-HCl gel. A 32-kDa band appeared at similar intensity in all the lanes loaded with the translation products (B) and the densitometric analysis (C).