Figure 10.

Differential X-OAT enzyme activities in ACs expressing β-gal, X-OAT-Mo, X-OATWT, and mutants. Messenger RNA (1 ng) from X-OATWT or mutants was injected into the animal pole at the two-cell stage. The ACs were dissected at stage 8.5 to 9.0 and cultured to equivalent of stage 22. The ACs were harvested in PBS buffer (16 ACs per group) for protein concentration measurement and X-OAT enzyme activity assay (see Methods). All groups were assayed in triplicate. P values are compared between β-gal and each OAT by Student's t-tests. *P < 0.01, compared with β-gal; **P < 0.001, compared with β-gal.