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Luciferase activity assay for the counter screen to identify false positives from the HTS of P23H mutant opsin clearance. (A) Coelenterazine h, catalyzed by recombinant Rluc, reacts with oxygen and generates coelenteramide h, CO2 and light that is read as luminescence. Inhibition of Rluc by a model compound causes a significant reduction of this luminescence. (B) The activity of Rluc treated with 0.1% DMSO or 200 μM Evans Blue. Error bars, SDs from 16 experiments.
