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. 2015 Apr 29;56(4):2553–2567. doi: 10.1167/iovs.14-16298

Figure 8.

Figure 8

The ALPHA immunoassay for confirmation of P23H mutant opsin clearance (left). (A) A standard curve of purified opsin fitted by the Hill function. Opsin standards were 0.0075, 0.025, 0.075, 0.25, 0.75, 2.5, 7.5, 25, 75, and 250 pg. (B) Quantification of P23H mutant opsin in lysates containing different numbers of cells. The value of 100% for a cell lysate was obtained from 10,000 cells cultured in a single well of a standard 384-well plate for 18 hours. Error bars represent SDs from experiments performed in triplicate.