Fig. 6.

The Focinator is a user friendly method that can be used without long term training. In Fig. 6, three different groups of users are compared. Programmers of the Focinator (n = 2), Biologist (n = 2) and users with no scientific background (n = 2) evaluated ten different cell lines. For each cell line about 80 pictures containing a total of about 500 nuclei were evaluated by the different users with the Focinator. The graph shows the calculated evaluation times per nucleus including a correction based on the numbers of pictures that had to be opened