Table 1.
An overview of the literature regarding oocytes retrieved from extracorporeal whole ovary tissue and subsequent IVM
| Transport from OK to lab | Transport from OK to lab | Collection method | In vitro maturation | [O2] | Duration | Strategy | Age (years) | No. of patients | No. of patients with COC | No. of COC | No. of COC for IVM | % maturation | % survival (vitrification) | No. of patients for ICSI/AA | No. of MII for ICSI/AA | % fertilization | No. of embryos vitrified | Conclusions | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Revel et al., 2003 | Leibovitz’s L-15 | Ice | Antral follicle puncture + medullar follicles | P-1 (Irvine) + SSS | 5 % | 24 + 24 h | Oocyte or embryo slow freezing | 14–31 | 9 | 7 | 32 | 10 | 50 % | 3 | 5 | 100 | 3 | Clinical case report | |
| Isachenko et al., 2004 | DPBS + 10 % HSA | 37 °C | Antral follicle puncture | IVF medium (IVF Science) + FSH + hCG + E2 + ITS + patient’s serum | 20 % | 36 h | Oocyte slow freezing | 26–33 | 2 | 2 | 18 | 18 | 56 % | Clinical case report | |||||
| Huang et al., 2007 | Leibovitz’s L-15 | 4 °C | Antral follicle puncture | IVM medium (Cooper Surgical) + FSH + LH | 20 % | 24 + 24 h | Oocyte vitrification | 43 | 1 | 1 | 4 | 4 | 75 % | Clinical case report | |||||
| Huang et al., 2008 | Leibovitz’s L-15 | 4 °C | Antral follicle puncture + medullar follicles | IVM medium (SAGE) + FSH + LH | 20 % | 24 + 24 h | Oocyte vitrification | 18–38 | 4 | 4 | 11 | 11 | 79 % | Clinical case report | |||||
| Huang et al., 2008b | Leibovitz’s L-15 | 4 °C | Antral follicles puncture + medullar follicles | IVM medium (SAGE) + FSH + LH | 20 % | 24 + 24 h | Oocyte vitrification | 16 | 1 | 1 | 11 | 11 | 73 % | Clinical case report: mosaic Turner syndrome | |||||
| Revel et al., 2009 | Leibovitz’s L-15 | Ice | Antral follicle puncture + medullar follicles | P1 (Irvine) + SSS or IVM medium (Sage) or homemade IVM medium | 5 % | 24 + 24 h | Oocyte slow freezing | 5–20 | 19 | 17 | 179 | 133 | 34 % | Retrospective clinical study: oocyte retrieval possible from 5 years onwards | |||||
| Gonzalez et al., 2011 | Leibovitz’s L-15 | Cold | Antral follicle puncture | IVM medium (Origio) + FSH + hCG | 20 % | 24 + 24 h | Oocyte vitrification | 28 | 1 | 1 | 2 | 2 | 100 % | Clinical case report | |||||
| Fasano et al., 2011 | Leibovitz’s L-15 | 4 °C | Antral follicle puncture + medullar follicles | IVM medium (SAGE) + FSH + LH | 20 % | 24 + 24 h | Oocyte or embryo vitrification | 8–35 | 57 | 42 | 266 | 235 | 31 % | 3 | 10 | – | 5 | Retrospective clinical study: oocyte retrieval possible regardless menstrual phase, use oral contraceptives or age | |
| Escriba et al., 2012 | DMEM | 25 °C | Antral follicle excision + medullar follicles | CCM (Vitrolife) | 20 % | 24 + 24 h | Artificial activation | 15–38 | 33 | 30 | 108 | 108 | 36 % | 41b | Research study: oocyte retrieval possible from follicles > and <6 mm, regardless menstrual phase | ||||
| Imesch et al., 2013 | IVM medium (Sage) | NA | Antral follicle puncture | IVM medium (SAGE) + FSH + LH | 20 % | 24 + 24 h | Oocyte vitrification + activation | 18–41 | 7 | 7 | 63 | 63 | 62 % | 62 % | 75b | Research study: in vitro maturation, oocyte vitrification and artifical activation | |||
| Wilken-Jensen et al.,2013 | N.A. | 37° 5–10′ or 0° 2–5 h | Antral follicle puncture + medullar follicles | LAG + IVM medium (Origio) + FSH/LH/EGF/FF in different compositions | 7 % | 24 + 24 h | Time lapse imaging of IVM | 0–38 | 69 | 61 | 682 | 682 | 3 %a | Research study: oocyte retrieval possible after transport, after chemo/radio therapy, but compromizes maturation rate. | |||||
| Prasath et al., 2013 | HTF | NA | Antral follicle puncture | IVM medium (Origio) + FSH + hCG | NA | 24 h | Embryo slow freezing | 21 | 1 | 1 | 4 | 4 | 100 % | 1 | 4 | – | 3 | Clinical case report: live birth | |
| Takae et al., 2014 | Saline solution | 37 °C | Antral follicle puncture + medullar follicles | IVM medium (Origio) + FSH + SSS | 5 % | 24 + 24 h | Oocyte cryopreservation? | 25–41 | 27 | 25 | 226 | 215 | 50 % | Retrospective clinical study: oocyte retrieval is correlated with AMH, retrieval regardless menstrual phase | |||||
| Shirasawa et al., 2013 | PBS | Warm | Antral follicle puncture | M199 + SSS + FSH | 5 % | 24 + 24 h | Oocyte fixation | 35–44 | 8 | 8 | 87 | 87 | 13 % | Research study: some spindle and chromatin abnormalities |
Literature overview of published research regarding oocytes retrieved from extracorporeal whole ovary tissue for cortex cryopreservation
NA not available, DPBS Dulbecco’s phosphate buffered saline, DMEM Dulbecco’s modified Eagle’s medium, HTF Human Tubal fluid, P-1 preimplantation stage 1, SSS serum substitute supplement, IVF in vitro fertilization, FSH follicle-stimulating hormone, hCG human chorionic gonadotropin, E2 estradiol, ITS insulin-transferrin-selenium, LH luteinizing hormone, CCM blastocyst culture medium, EGF epidermal growth factor, FF: Follicle Fluid, M199: Medium 199
aIncludes naked oocytes in IVM
bArtificial activation, not fertilization