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. 2015 Aug 26;10:5423–5434. doi: 10.2147/IJN.S90014

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© 2015 Lee et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Intracellular translocation of hBD3-3 in vitro.

Notes: (A) Cellular localization of rhodamine-labeled peptide fragments in RAW 264.7 cells. Cells (1×10⁴) were incubated for 10 minutes in medium containing the rhodamine-labeled peptides (50 μM) (original magnification 40×). (B) FACS analysis of cells treated with rhodamine-labeled peptides. Cells (1×10⁶) were incubated for 10 minutes and 30 minutes in medium containing the rhodamine-labeled peptides (50 μM).

Abbreviations: hBD3, human beta-defensin 3; FACS, fluorescence-activated cell sorting; DAPI, 4′,6-diamidino-2-phenylindole; min, minutes.