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. 2015 Apr 10;4(4):533–540. doi: 10.1002/mbo3.257

Figure 1.

Figure 1

The mechanism of riboregulator for target gene regulation and the scaffold-fused taRNA. (A) Expression of a target gene located downstream of the ribosome-binding site (RBS) of crRNA is induced in presence of taRNA, which exposes the RBS upon taRNA-crRNA hybridization. (B) Scaffold-fused taRNA harbors a sRNA scaffold sequence at the 3′-end. (C) Predicted secondary structure of sRNA scaffold sequences used in this study. The MicF scaffold is derived from natural Escherichia coli MicF sRNA. MicF M7.4 scaffold harbors GC-rich stem structures and a mutated loop region (noted in bold letters).